POLD3 Antibody

SKU: orb1256509

Description

POLD3 Antibody

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 197-466 of human POLD3 (NP_006582.1).
• Target: POLD3
• Molecular Weight: Observed: 51kDa
• Purification: Affinity purification

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

DNA polymerase delta subunit 3, DNA polymerase delta subunit p66, POLD3, KIAA0039

Western blot analysis of extracts of various cell lines, using POLD3 antibody (orb1256509) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Immunohistochemistry of paraffin-embedded human oophoroma using POLD3 antibody (orb1256509) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using POLD3 antibody (orb1256509). Green：GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining.RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.

Immunofluorescence analysis of U2OS cells using POLD3 antibody (orb1256509).

Quick Database Links

Gene Symbol

POLD3

UniProt

Q15054