POLB Rabbit Polyclonal Antibody

SKU: orb626429

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Description

Rabbit polyclonal antibody to DNA polymerase beta

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, IHC, IP, WB
Dilution Range	WB: 1:500-1:2000; IP: 1:200-1:1000; IHC: 1:20-1:200
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	polymerase(DNA directed), beta
Target	POLB
Molecular Weight	38 kDa
Purity	≥95% as determined by SDS-PAGE
Purification	Immunogen affinity purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	liquid
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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IP Result of anti-DNA polymerase beta (IP: orb626429, 4 ug; Detection: orb626429 1:800) with K-562 cells lysate 3200 ug.

K-562 cells were subjected to SDS PAGE followed by western blot with orb626429 (POLB antibody) at dilution of 1:1000.

Immunohistochemistry of paraffin-embedded human testis using orb626429 (POLB antibody) at dilution of 1:100.

Quick Database Links

Gene Symbol

POLB

UniProt

P06746

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Immunoprecipitation

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