POLA1 Antibody (C-Term)

• Catalog Number: orb1925436
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB57637
• Tested applications: FC, WB
• Predicted Reactivity: Human
• Reactivity: Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, FC: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 165913 Da
• Target: This POLA1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1406-1439 amino acids from the human region of human POLA1.
• UniProt ID: P09884
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: DNA polymerase alpha catalytic subunit, 2.7.7.7, D
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-POLA1 Antibody (C-Term) at 1:2000 dilution + NIH/3T3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 166 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing A431 cells stained (green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.