PNPLA3 Antibody (N-term)

SKU: orb1935005

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Metabolism Research

Images & Validation

• Tested Applications: WB
• Dilution Range: WB - 1:1000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD57707
• Immunogen: This PNPLA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-137 amino acids from the N-terminal region of human PNPLA3. Antigen Region: 109-137 aa.
• Target: PLPL3
• Molecular Weight: 52865 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Patatin-like phospholipase domain-containing protein 3, Acylglycerol O-acyltransferase, 231-, Adiponutrin, Calcium-independent phospholipase A2-epsilon, iPLA2-epsilon, PNPLA3, ADPN, C22orf20

PNPLA3 Antibody (N-term) western blot analysis in K562 cell line lysates (35 ug/lane). This demonstrates the PNPLA3 antibody detected the PNPLA3 protein (arrow).

All lanes: Anti-PNPLA3 Antibody (N-term) at 1:2000 dilution. Lane1: mouse heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 46 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PNPLA3 Antibody (N-term) at 1:1000 dilution. Lane 1: mouse heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 46 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PNPLA3 Antibody (N-term) at 1:2000 dilution. Lane 1: mouse heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 46 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PNPLA3 Antibody (N-term) at 1:2000 dilution. Lane 1: mouse heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 46 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PNPLA3 Antibody (N-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 53 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

PLPL3

UniProt

Q9NST1

RefSeq (Protein):NP_079501.2