PNKP Antibody

• Catalog Number: orb1256844
• Category: Antibodies
• Description: PNKP Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 60-320 of human PNKP (NP_009185.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 57kDa
• Target: PNKP
• UniProt ID: Q96T60
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: PNKP, polynucleotide kinase 3'-phosphatase, PNK, p
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using PNKP antibody (orb1256844) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat kidney using PNKP antibody (orb1256844) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using PNKP antibody (orb1256844) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human prostate cancer using PNKP antibody (orb1256844) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse liver using PNKP antibody (orb1256844) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using PNKP antibody (orb1256844) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using PNKP antibody (orb1256844). Green：GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.