PMS2 Antibody

• Catalog Number: orb1260298
• Category: Antibodies
• Description: PMS2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 390-670 of human PMS2 (NP_000526.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 120kDa
• Target: PMS2
• UniProt ID: P54278
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: PMS2, HNPCC4, H_DJ0042M02.9, PMS2CL, PMSL2, PMS1 p
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using PMS2 antibody (orb1260298) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunofluorescence analysis of U2OS cells using PMS2 antibody (orb1260298).

Immunoprecipitation analysis of 150 ug extracts of Jurkat cells using 3 ug PMS2 antibody (orb1260298). Western blot was performed from the immunoprecipitate using PMS2 antibody (orb1260298) at a dilution of 1:500.