PML Sumoylation Site Antibody

SKU: orb33903

Description

Purified rabbit polyclonal antibody.

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:50-100, IF/ICC: 1:10-50, FACS: 1:10-50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB2570
• Immunogen: Synthetic Peptide
• Target: PML
• Molecular Weight: 97551

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

Anti-Tripartite motif-containing protein 19 antibody, anti-RING finger protein 71 antibody, anti-MYL antibody, anti-RNF71 antibody, anti-TRIM19 antibody

Western blot analysis of anti-PML Sumoylation Site Antibody in Hela cell line lysates (35 ug/lane). PML (arrow) was detected using the purified Pab.

PML Sumoylation Site Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of PML Sumoylation Site Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Quick Database Links

Gene Symbol

PML

UniProt

P29590

RefSeq (Protein):NP_150250.2, NP_150241.2, NP_150242.1, NP_150252.1, NP_150253.2, NP_150243.2, NP_150247.2, NP_150249.1, NP_002666.1