PML Antibody, KO Validated

• Catalog Number: orb1274530
• Category: Antibodies
• Description: PML Antibody, KO Validated
• Target: PML
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 300-600 of human PML (NP_150253.2).
• UniProt ID: P29590
• MW: Observed: 70-130kDa
• Tested applications: IF, IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PML, MYL, PP8675, RNF71, TRIM19
• Note: For research use only

Western blot analysis of extracts of various cell lines, using PML antibody (orb1274530) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of extracts from normal (control) and PML knockout (KO) HeLa cells, using PML antibody (orb1274530) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human gastric cancer using PML antibody (orb1274530) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human uterine cancer using PML antibody (orb1274530) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using PML antibody (orb1274530). Blue: DAPI for nuclear staining.