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Catalog Number | orb1930463 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB21251 |
Tested applications | FC, IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 18679 Da |
Target | This PMCH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 94-122 amino acids from the Central region of human PMCH. |
UniProt ID | P20382 |
NCBI | NP_002665.2 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Pro-MCH, Neuropeptide-glycine-glutamic acid, NGE, Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
PMCH Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
PMCH Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PMCH Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
PMCH Antibody (Center) western blot analysis in K562 cell line lysates (15 ug/lane). This demonstrates the PMCH antibody detected the PMCH protein (arrow).
PMCH Antibody (Center) western blot analysis in mouse brain tissue lysates (15 ug/lane). This demonstrates the PMCH antibody detected the PMCH protein (arrow).
Western blot analysis of PMCH (arrow) using rabbit polyclonal PMCH Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PMCH gene.