PLOD1 Antibody (N-term)

• Catalog Number: orb1788099
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: 66-94 aa
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, FC: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 83550 Da
• Target: This PLOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-94 amino acids from the N-terminal region of human PLOD1.
• UniProt ID: Q02809
• NCBI: NP_000293.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-PLOD1 Antibody (N-term) at 1:1000 dilution. Lane 1: A431 whole cell lysates. Lane 2: Hela whole cell lysates. Lane 3: K562 whole cell lysates. Lane 4: MCF-7 whole cell lysates. Lane 5: U-87MG whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 84 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing U-87 MG cells stained (green line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.