PLK3 Antibody

• Catalog Number: orb1535151
• Category: Antibodies
• Description: PLK3 Antibody
• Target: PLK3
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: Peptide with sequence PDRLPISSCVTVPD, from the internal region of the protein sequence according to NP_004064.2.
• Tested applications: ELISA, IHC, IHC-P, WB
• Dilution range: IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (1 - 3 µg/ml)
• Application notes: Further information: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 70kD band observed in lysates of cell line HeLa (calculated MW of 71.6kD according to NP_004064.2). Recommended concentration: 1-3 ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PLK3, CNK, FNK, Polo-like kinase 3, PRK, Prolifera
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

PLK3 antibody (2 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.