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PLIN2 Recombinant Rabbit Monoclonal Antibody

SKU: orb1499344
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Description

PLIN2 Recombinant Rabbit Monoclonal Antibody

Images & Validation

Tested ApplicationsFC, IF, IHC-Fr, IHC-P, WB
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1:50-100
ReactivityHuman, Mouse, Rat
Predicted ReactivityHuman, Mouse

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityRecombinant
IsotypeIgG
Clone No.7B2
ImmunogenA synthesized peptide derived from human Perilipin 2 (1-69aa)
TargetPLIN2
Molecular Weight48 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
DisclaimerFor research use only

Alternative Names

PLIN2_HUMAN; Perilipin-2; ADFP; Adipophilin; Adipose differentiation-related protein;

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PLIN2 Recombinant Rabbit Monoclonal Antibody

Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 2 hours at room temperature, Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: Mouse brain, 2: Rat brain, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 48 kDa, Observed MW: 48 kDa.

PLIN2 Recombinant Rabbit Monoclonal Antibody

Cell line: HepG2, Fixation: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:50, Primary Ab incubation condition: 4°C, overnight, Secondary Ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1499344.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IHC-Fr
Immunohistochemistry Frozen
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
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PLIN2 Recombinant Rabbit Monoclonal Antibody (orb1499344)

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Available Sizes

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25 μl
$ 170.00
50 μl
$ 320.00
100 μl
$ 460.00
DispatchUsually dispatched within 5-10 working days
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