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PLEC Antibody (C-Term)
Catalog Number: orb1925510
| Catalog Number | orb1925510 |
|---|---|
| Category | Antibodies |
| Description | Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB56747 |
| Tested applications | IF, WB |
| Predicted Reactivity | Hamster, Mouse, Rat |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | IF: 1:25, WB: 1:1000 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 531791 Da |
| Target | This PLEC antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 4241-4275 amino acids from human PLEC. |
| UniProt ID | Q15149 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Plectin, PCN, PLTN, Hemidesmosomal protein 1, HD1, Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Anti-PLEC Antibody (C-Term) at 1:1000 dilution + U-2OS whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 532 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling PLEC at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
