PLD2 Antibody (N-term)

• Catalog Number: orb1935594
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB34393
• Tested applications: WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, WB: 1:1000, WB: 1:2000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 105987 Da
• Target: This PLD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-39 amino acids from the N-terminal region of human PLD2.
• UniProt ID: O14939
• NCBI: NP_002654.3, NP_001230037.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Phospholipase D2, PLD 2, hPLD2, Choline phosphatas
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

PLD2 Antibody (N-term) western blot analysis in MDA-MB453 cell line lysates (35 ug/lane). This demonstrates the PLD2 antibody detected the PLD2 protein (arrow).

PLD2 Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35 ug/lane). This demonstrates the PLD2 antibody detected the PLD2 protein (arrow).

Western blot analysis of lysate from human heart tissue lysate, using PLD2 Antibody (N-term). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.

Anti-PLD2 Antibody (N-term) at 1:2000 dilution + Mouse lung tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 106 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.