PLCB1 Antibody

• Catalog Number: orb1258361
• Category: Antibodies
• Description: PLCB1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 917-1216 of human PLCB1 (NP_056007.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 139kDa
• Target: PLCB1
• UniProt ID: Q9NQ66
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: 1-phosphatidylinositol 4,5-bisphosphate phosphodie
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of NCI-H460 cells, using PLCB1 antibody (orb1258361) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded rat heart using PLCB1 antibody (orb1258361) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human kidney cancer using PLCB1 antibody (orb1258361) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using PLCB1 antibody (orb1258361) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using PLCB1 antibody (orb1258361). Blue: DAPI for nuclear staining.