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| Catalog Number | orb769480 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to PLC gamma 1 (phospho-Tyr1253) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PLCG1 around the phosphorylation site of Tyr1253. AA range:1221-1270 |
| MW | 150 |
| Tested applications | ELISA, WB |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Anti-PLCG1 antibody, anti-PLC1 antibody, anti-1-ph Read more... |
| Note | For research use only |
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PLCG1 (Phospho-Tyr1253) Antibody.
Western blot analysis of lysates from LOVO cells treated with and 293 cells treated with heat shock, using PLCG1 (Phospho-Tyr1253) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
