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Catalog Number | orb1252649 |
---|---|
Category | Antibodies |
Description | Plasma Cell Marker Antibody [LIV3G11] |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | LIV3G11 |
Tested applications | IHC |
Reactivity | Human |
Isotype | IgG2a |
Immunogen | Pancreatic cancer-related mucin was used as the immunogen for this Plasma cell marker antibody. |
Concentration | 0.2 mg/mL |
Dilution range | IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)NOT suitable for frozen tissue stainingThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the marker antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Note | For research use only |
Application notes | IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)NOT suitable for frozen tissue stainingThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the marker antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
FFPE human tonsil stained with plasma cell marker antibody (LIV3G11).
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
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