PKLR Antibody (N-term)

SKU: orb1929614

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Metabolism Research

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:50-100
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD25814
• Immunogen: This PKLR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PKLR. Antigen Region: 1-30 aa.
• Target: PKLR
• Molecular Weight: 61830 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Pyruvate kinase PKLR, Pyruvate kinase 1, Pyruvate kinase isozymes L/R, R-type/L-type pyruvate kinase, Red cell/liver pyruvate kinase, PKLR, PK1, PKL

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

The anti-PKLR Pab is used in Western blot to detect PKLR in NIH/3T3 cell lysate.

PKLR Antibody (S8) western blot analysis in NCI-H292 cell line lysates (35 ug/lane). This demonstrates the PKLR antibody detected the PKLR protein (arrow).

Quick Database Links

Gene Symbol

PKLR

UniProt

P30613

RefSeq (Protein):NP_000289.1, NP_870986.1