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PKC Recombinant Rabbit Monoclonal Antibody
Description
Images & Validation
−| Tested Applications | FC, ICC, IF, IHC-Fr, IHC-P, IP, WB |
|---|---|
| Dilution range | WB=1:500-2000, ICC/IF=1:50-200, IP=1:20-50, Flow-Cyt=1:50-100, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200 |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | 11H15 |
| Immunogen | A synthesized peptide derived from human PKC (300-500/671aa) |
| Target | PRKCG |
| Molecular Weight | 77 kDa |
| Purification | Affinity purified by Protein A |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% rAlbumin, 0.02% Proclin300 and 50% glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |
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Western blot analysis of (1) HeLa cell lysate, (2) A431 cell lysate. Using PKC (orb2561153) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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PKC Recombinant Rabbit Monoclonal Antibody (orb2561153)
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