PKC α (phospho Tyr658) rabbit pAb Antibody

SKU: orb769636

Description

Rabbit polyclonal antibody to PKC alpha (phospho-Tyr657)

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB 1:500-2000;IHC-p 1:50-300
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human PKC alpha around the phosphorylation site of Tyr657. AA range:623-672
• Molecular Weight: 80
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-PRKCA antibody, anti-PKCA antibody, anti-PRKACA antibody, anti-Protein kinase C alpha type antibody, anti-PKC-A antibody, anti-PKC-alpha antibody

Western Blot analysis of 293 cells using Phospho-PKC α (Y657) Polyclonal Antibody diluted at 1:1000.

Western blot analysis of lysates from COLO205 cells, using PKC alpha (Phospho-Tyr657) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human Breast cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).