PKC δ (phospho Tyr52) rabbit pAb

• Catalog Number: orb769650
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PKC delta (phospho-Tyr52)
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human PKC delta around the phosphorylation site of Tyr52. AA range:18-67
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 77
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-PRKCD antibody, anti-Protein kinase C delta t
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKC delta (Phospho-Tyr52) Antibody.

Immunofluorescence analysis of NIH/3T3 cells, using PKC delta (Phospho-Tyr52) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using PKC delta (Phospho-Tyr52) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from Jurkat cells treated with starved 24h, using PKC delta (Phospho-Tyr52) Antibody. The lane on the right is blocked with the phospho peptide.