PKC alpha Rabbit mAb [KD Validated]

• Catalog Number: orb1951751
• Category: Antibodies
• Description: PKC alpha is an AGC kinase of the PKC family. A classical PKC downstream of many mitogenic and receptors. Classical PKCs are calcium-dependent enzymes that are activated by phosphatidylserine, diacylglycerol and phorbol esters.
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: FC, ICC, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human PKC alpha
• Antibody Type: Recombinant Antibody
• Dilution range: WB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:50
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Conjugation: Unconjugated
• UniProt ID: P17252
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: EC 2.7.11.13; KPCA; PKC III; PKC-A; PKC-III; PKC-a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Immunofluorescent analysis of Hela cells, using PKC alpha Antibody.

Immunohistochemical analysis of paraffin-embedded human colon, using PKC alpha Antibody.

Western blot analysis of PKC alpha expression in HEK293 cell lysate.