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Description
Research Area
Images & Validation
−| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | PKA alpha/beta CAT (phospho Thr197) antibody was raised against a peptide sequence around phosphorylation site of threonine 197 (R-T-W (p)-T-L) derived from Human PKA alpha/beta CAT. |
| Target | PRKACA |
| Molecular Weight | 40 kDa |
| Purification | Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Concentration | 1 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PKA alpha/beta CAT (Phospho-Thr197) antibody (left) or the same antibody preincubated with blocking peptide (right).

Immunofluorescence staining of methanol-fixed A549 cells using PKA alpha/beta CAT (Phospho-Thr197) Antibody.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_002721.1 |
|---|
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Protocol Information
PRKACA Antibody (orb1253150)
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