PITX3 polyclonal antibody

SKU: orb1089066

Description

PITX3 polyclonal antibody

Research Area

Developmental Biology, Epigenetics

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: IHC-P=1:100-500 (Paraffin sections require antigen retrieval.), not yet tested in other applications, optimal dilutions/concentrations should be determined by the end user.
• Reactivity: Bovine, Canine, Equine, Gallus, Human, Mouse, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide derived from human PITX3:231-302/302
• Molecular Weight: ~32kDa
• Purification: affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Homeobox protein PITX 3 antibody, anti-Homeobox protein PITX3 antibody, anti-MGC12766 antibody, anti-Paired like homeodomain transcription factor 3 antibody, anti-Paired-like homeodomain transcription factor 3 antibody, anti-Pituitary homeobox 3 antibody, anti-PITX 3 antibody, anti-Pitx3 antibody, anti-PITX3_HUMAN antibody, anti-PTX 3 antibody, anti-PTX3. antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen re- trieval by boiling in sodium citrate buffer (pH6.0) for 15min. Block en- dogenous peroxidase by 3% hydrogen peroxide for 20 minutes. Block- ing buffer (normal goat serum) at 37°C for 30min. Antibody incubation with PITX3 polyclonal antibody (orb1089066), unconjugated at 1:400 overnight at 4°C.

Paraformaldehyde-fixed, paraffin embedded (Rat eye); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endoge- nous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PITX3 polyclonal antibody (orb1089066), unconjugated at 1:400 overnight at 4°C.

Quick Database Links

UniProt

O75364