PITPNA Antibody (C-term)

• Catalog Number: orb1788381
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: 243-270 aa
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:2000, WB: 1:2000, IHC-P: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 31806 Da
• Target: This PITPNA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-270 amino acids from the C-terminal region of human PITPNA.
• UniProt ID: Q00169
• NCBI: NP_006215.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Phosphatidylinositol transfer protein alpha isofor
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded H. skin section using PITPNA Antibody (C-term). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Western blot analysis of lysates from Hela cell line, mouse brain, mouse cerebellum tissue, mouse NIH/3T3, rat PC-12 cell line, rat brain, rat cerebellum tissue, U-251 MG cell line (from left to right), using PITPNA Antibody (C-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

All lanes: Anti-PITPNA Antibody (C-term) at 1:2000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: MOLT-4 whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: U-251 MG whole cell lysate. Lane 5: mouse brain lysate. Lane 6: NIH/3T3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 32 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-PITPNA Antibody (C-term) at 1:2000 dilution. Lane 1: NIH/3T3 whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: MOLT-4 whole cell lysate. Lane 5: U-251 MG whole cell lysate. Lane 6: mouse brain lysate. Lane 7: rat brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 32 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.