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Catalog Number | orb1928656 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB1753 |
Tested applications | IHC-P, WB |
Predicted Reactivity | Rat |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, IHC-P: 1:50~100, WB: 1:1000 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 47300 Da |
Target | This PIP5K2G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 333-364 amino acids from the C-terminal region of human PIP5K2G. |
UniProt ID | Q8TBX8 |
NCBI | NP_001139730.1, NP_001139732.1, NP_001139731.1, NP_079055.3 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Phosphatidylinositol 5-phosphate 4-kinase type-2 g Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of anti-PIP5K2G Pab in mouse brain cell lysate. PIP5K2G (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot analysis of lysate from human brain tissue lysate, using PIP5K2G Antibody (E348). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug per lane.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.