PIP4K2B Antibody

SKU: orb345754

Description

PIP5K2B antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: ELISA: 1:20,000 - 1:60,000, IHC: 1:100-1:500, IF: 1:100-1:500, WB: 0.2 µg/ml
• Reactivity: Gallus, Human, Mouse, Primate, Rat
• Application Notes:
  Anti-PIP5K2B antibody is tested for ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~54 kDa corresponding to the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: PIP5K2B affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of human PIP5K2B.
• Target: PIP4K2B
• Purity: Anti-PIP5K2B was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific towards PIP5K2B. A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, Rat, Chicken, and Primate based on 100% sequence homology. Cross-reactivity with PIP5K2B from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: None. Stabilizer: 50% (v/v) Glycerol; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.65 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-PIP5K2B antibody, Phosphatidylinositol 5-phosphate 4-kinase type-2 beta, 1-phosphatidylinositol 5-phosphate 4-kinase 2-beta, Diphosphoinositide kinase 2-beta, Phosphatidylinositol 5-phosphate 4-kinase type II beta, PI(5)P 4-kinase type II beta, PIP4KII-beta, PtdIns(5)P-4-kinase isoform 2-beta, PIP4K2B, PIP5K2B

Western Blot of Rabbit Anti-PIP5K2B Antibody. Lane 1: Mouse Brain homogenate samples. Load: 10 µg per lane. Primary antibody: PIP5K2B antibody at 0.2 µg/ml for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:40000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 54 kDa for PIP5K2B. Other band(s): None.

Quick Database Links

Gene Symbol

PIP4K2B

UniProt

Q8TCG2