PIP4K2A Antibody (C-term)

• Catalog Number: orb1926738
• Category: Antibodies
• Description: PIP4K2A Antibody (C-term)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Porcine
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P48426
• MW: 46225 Da
• Tested applications: FC, IF, IHC-P, WB
• Dilution range: IF - 1:25, IHC-P - 1:100-500, FC - 1:25, WB - 1:1000
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Research Area: Metabolism Research, Signal Transduction
• Note: For research use only

Western blot analysis of lysate from human brain tissue, using PIP4K2A Antibody (C-term). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.

Immunohistochemical analysis of paraffin-embedded H. brain section using PIP4K2A Antibody (C-term). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Flow cytometric analysis of Hela cells using PIP4K2A Antibody (C-term) (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling PIP4K2A at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing cytoplasmic staining on Hela cell line. Cytoplasmic actin is detected with Alexa Fluor 555 conjugated with Phalloidin at 1/100 dilution (red).