PINX-1 Polyclonal Antibody

• Catalog Number: orb1416338
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PINX-1.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-Fr, IHC-P, WB
• Reactivity: Porcine, Rat
• Conjugation: Unconjugated
• UniProt ID: Q96BK5
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: PIN2/TERF1-interacting telomerase inhibitor 1, Liv
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-PINX-1 Polyclonal Antibody, Unconjugated 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Blank control: Mouse spleen.Primary Antibody (green line): Rabbit Anti-PINX-1 antibody.Dilution: 2 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647. Dilution: 1 µg /test.Protocol. The cells were fixed with 4% PFA (10min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at-20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20, 000 events was performed