PINK1 Antibody

SKU: orb1238781

Description

PINK1 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  PINK1 antibody can be used for detection of PINK1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: PINK1 antibody was raised against a 16 amino acid peptide near the amino terminus of human PINK1.The immunogen is located within amino acids 120 - 170 of PINK1.
• Target: PINK1
• Molecular Weight: Predicted: 55 kDa Observed: 53 kDa
• Purification: PINK1 antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PINK1 antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PINK1 Antibody: BRPK, PARK6BRPK

Western blot analysis of PINK1 in A431 cell lysate with PINK1 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.

Immunofluorescence of PINK1 in mouse brain tissue with PINK1 Antibody at 20 µg/mL. Green: PINK1 antibody (orb1238781) Red: Phylloidin staining Blue: DAPI staining.

Western blot analysis of PINK1 in (A) mouse and (B) rat spleen tissue lysate with PINK1 antibody at 1 µg/mL.

Quick Database Links

Gene Symbol

PINK1

UniProt

Q9BXM7

RefSeq (Protein):NP_115785