PINK1 Antibody

• Catalog Number: orb1238781
• Category: Antibodies
• Description: PINK1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: PINK1 antibody was raised against a 16 amino acid peptide near the amino terminus of human PINK1.The immunogen is located within amino acids 120 - 170 of PINK1.
• Concentration: 1 mg/mL
• Dilution range: PINK1 antibody can be used for detection of PINK1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 55 kDa Observed: 53 kDa
• Target: PINK1
• UniProt ID: Q9BXM7
• NCBI: NP_115785
• Storage: PINK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
• Buffer/Preservatives: PINK1 antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: PINK1 Antibody: BRPK, PARK6BRPK
• Note: For research use only
• Application notes: PINK1 antibody can be used for detection of PINK1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of PINK1 in A431 cell lysate with PINK1 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.

Immunofluorescence of PINK1 in mouse brain tissue with PINK1 Antibody at 20 µg/mL. Green: PINK1 antibody (orb1238781) Red: Phylloidin staining Blue: DAPI staining.

Western blot analysis of PINK1 in (A) mouse and (B) rat spleen tissue lysate with PINK1 antibody at 1 µg/mL.