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Catalog Number | orb1262436 |
---|---|
Category | Antibodies |
Description | PIN1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IF, IHC-P, WB |
Predicted Reactivity | Monkey |
Reactivity | Human |
Isotype | Rabbit Ig |
Immunogen | This PIN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-56 amino acids from the Central region of human PIN1. |
Concentration | batch dependent |
Dilution range | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 18 kDa |
Target | PIN1 |
UniProt ID | Q13526 |
NCBI | Q13526 |
Storage | Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Peptidyl-prolyl cis-trans isomerase NIMA-interacti Read more... |
Note | For research use only |
Application notes | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of PIN1 Antibody in HL-60 cell line lysates (35 ug/lane)
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PIN1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
PIN1 Antibody FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of PIN1 using rabbit polyclonal PIN1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIN1 gene.
Confocal immunofluorescent analysis of PIN1 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
ICC, IHC-Fr, IHC-P, IP, WB | |
Hamster, Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Porcine, Primate, Rat, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
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