PIK3R2 Antibody

SKU: orb1410057

Description

Mouse monoclonal antibody to PIK3R2

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Infectious Disease & Virology, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IHC
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: PIK3R2/292
• Immunogen: Recombinant fragment of human PIK3R2 protein (around aa 520-720) (exact sequence is proprietary)
• Target: PIK3R2
• Source/Expression System: Bioreactor
• Molecular Weight: 85kDa
• Purification: Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

p85 beta; P85B; Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 2 (p85 beta); Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; Phosphoinositide 3 kinase regulatory subunit polypeptide 2 (p85 beta); PI3 kinase p85 beta subunit; PI3K regulatory subunit beta; PIK3R2; PtdIns-3-kinase regulatory subunit p85-beta

Analysis of Protein Array containing more than 19000 full-length human proteins using PIK3R2-Monospecific Mouse Monoclonal Antibody (PIK3R2/292). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with PIK3R2 Mouse Monoclonal Antibody (PIK3R2/292). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human spleen stained with PIK3R2 Mouse Monoclonal Antibody (PIK3R2/292). HIER: Tris/EDTA, pH9.0, 45 min. 2°: HRP-polymer, 30 min. DAB, 5 min.

Quick Database Links

Gene Symbol

PIK3R2

UniProt

O00459