PIK3CB antibody

• Catalog Number: orb420351
• Category: Antibodies
• Description: PIK3CB antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This affinity purified PIK3C-ß antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an N-terminal region of human Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform.
• Concentration: 1.09 mg/ml
• Dilution range: ELISA: 1:50,000, WB: 1.0 ug/mL
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-PIK3C beta was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for human PIK3C beta protein. A BLAST analysis was used to suggest cross-reactivity with PI3K-beta based on a 100% homology from human sources with the immunizing sequence. Expect partial cross reactivity to PIK3C beta from mouse and rat (93% homology) sources. Cross-reactivity with PIK3C beta from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P42338
• NCBI: NP_006210.1
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-PIK3CB antibody, rabbit anti-PI3K beta
• Note: For research use only
• Application notes: Anti-PIK3C beta Antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 122.7 kDa in size corresponding to PI3K beta by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit anti-PIK3CB antibody. Marker: Opal Pre-stained ladder. Lane 1: HCT-116 WCL (p/n orb348712). Lane 2: MDA-MB-435S WCL (p/n orb348678). Load: 35 µg per lane. Primary antibody: PIK3CB antibody at 1:1000 for overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:30000 for 60 min at RT. Blocking Buffer: (p/n orb348637) for 30 min at RT. Predicted/Observed size: 122 kDa for PIK3CB.

Western Blot of Rabbit anti-PIK3CB antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate (p/n orb348669). Lane 2: MDA-MB-435S WCL (p/n orb348678). Load: 35 µg per lane. Primary antibody: PIK3CB antibody at 1:1000 for 3hrs at RT. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:30000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 122 kDa for PIK3CB.