PIK3CA Antibody

SKU: orb1247102

Description

PIK3CA Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments in lysates of cell lines A431, HeLa and Jurkat gave no specific signal but low background (at antibody concentration up to 1µg/mL).Immunohistochemistry:In paraffin embedded Human Brain (Cerebral Cortex) shows cytoplasm stained in the cell bodies. Recommended concentration: 2-3µg/mL. Paraffin embedded Human Heart. Recommended concentration: 5µg/mL.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-HQWLKDKNKGEIYD
• Target: PIK3CA
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PIK3CA, p110-alpha, phosphoinositide-3-kinase, catalytic, alpha polypeptide , MGC142161, MGC142163, PI3K, PI3-kinase p110 subunit alpha, PtdIns-3-kinase p110, phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha, phosphatidylinositol 3-kinase, catalytic, alpha polypeptide, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform

Quick Database Links

Gene Symbol

PIK3CA

UniProt

P42337

RefSeq (Protein):NP_006209.2