PIK3CA Antibody (HRP)

SKU: orb414120

Description

Rabbit polyclonal antibody against PIK3CA conjugated to HRP

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

• Tested Applications: WB
• Dilution range: WB (1:1000)
• Reactivity: Mouse
• Application Notes:
  A 1:1000 dilution of SPC-741 was sufficient for detection of PIK3CA in 15 µg of mouse brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of Human PIK3CA (150-250 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
• Target: PIK3CA
• Molecular Weight: 55.7 kDa
• Purification: Peptide Affinity Purified
• Conjugation: HRP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide, 0.23mM Citrate
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, PI3K alpha, PI3K-alpha, PI3K, p110 alpha, p110alpha, PI3 kinase subunit alpha, PI3 kinase p110 subunit alpha, PtdIns 3 kinase subunit p110 alpha, PtdIns-3-kinase subunit p110-alpha, PtdIns-3-kinase subunit alpha, PIK3CA, EC:2.7.1.153, EC:2.7.11.1, PI 3 Kinase catalytic subunit alpha, Phosphoinositide 3 kinase catalytic alpha polypeptide, PtdIns 3 kinase regulatory subunit alpha, Phosphatidylinositol 3 kinase regulatory subunit polypeptide 1, GRB1, Phosphoinositide 3 kinase regulatory subunit 1 (alpha), Phosphatidylinositol 3 kinase regulatory subunit p85 alpha, Phosphatidylinositol 3 kinase regulatory 1, PIK3R1, PI3-kinase subunit alpha, Phosphatidylinositol 3 kinase catalytic 110 KD alpha, PI3 kinase regulatory subunit alpha, p85 alpha, PI3Kalpha, Phosphoinositide 3 kinase regulatory subunit 1, MGC142161, MGC142163, Serine/threonine protein kinase PIK3CA, 5-bisphosphate 3-kinase catalytic subunit alpha isoform, Phosphatidylinositol 3 kinase associated p 85 alpha, p85, Phosphatidylinositol 3 kinase 85 kDa regulatory subunit alpha, PI3 kinase subunit p85 alpha, PtdIns 3 kinase subunit alpha

Western blot analysis of Mouse brain lysate showing detection of ~55.7 kDa PIK3CA protein using Rabbit Anti-PIK3CA Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain lysate. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-PIK3CA Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~55.7 kDa.

Quick Database Links

Gene Symbol

PIK3CA

UniProt

P42336

NCBI Gene ID

5290

RefSeq (Protein):NP_006209.2