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Catalog Number | orb1247103 |
---|---|
Category | Antibodies |
Description | PIK3C2A Antibody |
Target | PIK3C2A |
Clonality | Polyclonal |
Conjugation | Unconjugated |
Reactivity | Human |
Predicted Reactivity | Canine, Mouse |
Form/Appearance | Liquid |
Concentration | 500 ug/mL |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Immunogen | The immunogen for this antibody is: C-TVKWYQLTAATYL |
UniProt ID | O00443 |
Tested applications | ELISA, IHC |
Application notes | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave no signal but low background in Human Testis and Molt-4 lysates at up to 1ug/ml.Immunohistochemistry:In paraffin embedded Human Placenta shows cytoplasm staining of cytotrophoblasts and mesenchymal cells. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Heart. Recommended concentration: 5ug/ml. |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | PIK3C2A, CPK, PI3-K-C2A, PI3K-C2alpha, PI3-K-C2(AL Read more... |
Note | For research use only |
NCBI | NP_002636.2 |
orb1247103 (2 ug/ml) staining of paraffin embedded Human Placenta. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
orb1247103 (5 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ELISA, IHC | |
Canine, Human, Mouse | |
Goat | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Canine, Equine, Mouse, Porcine, Rabbit, Rat, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |