Pifithrin-α, p-Nitro, Cyclic

SKU: orb1309244

Description

Pifithrin-α, p-Nitro, Cyclic (PFN-α) is a cell-permeable, active-form inhibitor of the p53 tumor suppressor protein. It is widely used in research to transiently inhibit p53-dependent apoptosis and cell cycle arrest, facilitating studies in neurobiology, oncology, and ischemia-reperfusion injury across both in vitro and in vivo models.

Research Area

Cell Biology

Key Properties

• CAS Number: 60477-38-5
• MW: 299.35
• Purity: 99.43% (May vary between batches)
• Formula: C₁₅H₁₃N₃O₂S
• SMILES: [O-][N+](=O)C1=CC=C(C=C1)C1=CN2C(SC3=C2CCCC3)=N1
• Target: MDM-2/p53,p53
• Solubility: DMF:12.5 mg/mL (41.76 mM);DMSO:5 mg/mL (16.7 mM)

Bioactivity

• In Vivo:
  Intraocular administration of pifithrin-α at a concentration of 6 µM modestly enhances the survival of retinal ganglion cells (RGC), while it remains ineffective at 0.06 µM. Despite the observed in vitro benefits, pifithrin-α, p-Nitro, Cyclic (PFN-α) demonstrates no in vivo efficacy, even at the 6 µM concentration. Furthermore, pifithrin-α, p-Nitro, Cyclic exhibits a half-life (t1/2) of 6 hours under biological conditions.
  Read more...
• In Vitro:
  Pifithrin-α, p-Nitro, Cyclic (PFN-α) significantly inhibits p53-induced cell death and p21/WAF1 expression in cortical neurons exposed to etoposide, requiring concentrations an order of magnitude lower than PFT-α.
  Read more...

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

cell, cortical, P53, p53-triggered, neurons, inhibit, Inhibitor, MDM-2/p53, death, expression, Pifithrin α, p Nitro, Cyclic, Pifithrin-a, p-Nitro, Cyclic, Pifithrin-alpha, p-Nitro, Cyclic, Pifithrinα, pNitro, Cyclic, Pifithrin-α, p-Nitro, Cyclic, p21, WAF1, PFN-a, PFN-alpha, PFN-α, posttranscriptional

Compound Identifiers

PubChem CID

4587369