PICK1 Antibody

• Catalog Number: orb1247922
• Category: Antibodies
• Description: PICK1 Antibody
• Target: PICK1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-HYNKLQADPKQ
• UniProt ID: Q9NRD5
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 50kDa band observed in Mouse Brain lysates (calculated MW of 46.6kDa according to NP_036539.1, NP_001034672.1and NP_001034673.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Colon and Prostate. Recommended concentration: 5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PICK1, protein interacting with PRKCA 1, MGC15204,
• Research Area: Neuroscience, Signal Transduction
• Note: For research use only
• NCBI: NP_036539.1

orb1247922 (1 ug/ml) staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247922 (5 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247922 (5 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.