Piccolo Antibody (PerCP)

SKU: orb152172

Description

Rabbit polyclonal to Picollo (PerCP). Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains. In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1..

Images & Validation

• Tested Applications: IHC, WB
• Dilution range: WB (1:1000), IHC (1:400)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SPC-197 was sufficient for detection of Piccolo in 10 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat ant rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant Full length Protein, BC001304.1
• Target: Piccolo
• Molecular Weight: 550kDa
• Purification: Protein A Purified
• Conjugation: PerCP

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Protein piccolo, Aczonin, PCLO, Presynaptic cytomatrix protein, Multidomain presynaptic cytomatrix protein, Piccolo (presynaptic cytomatrix protein), PCLO_HUMAN, ACZ, Pico, Brain-derived HLMN protein, DKFZp779G1236, KIAA0559, Piccolo mouse homolog of Pico

Immunohistochemistry analysis using Rabbit Anti-Piccolo Polyclonal Antibody. Tissue: Muscle. Species: Mouse. Primary Antibody: Rabbit Anti-Piccolo Polyclonal Antibody at 1:400. Secondary Antibody: Alexa Fluor 488 Goat Anti-Rabbit. Counterstain: BTX (red). Localization: Selective staining of the NMJ.

Western blot analysis of Mouse, Rat brain cell lysates showing detection of Piccolo protein using Rabbit Anti-Piccolo Polyclonal Antibody. Primary Antibody: Rabbit Anti-Piccolo Polyclonal Antibody at 1:1000.

Quick Database Links

Gene Symbol

Piccolo

UniProt

Q9Y6V0

NCBI Gene ID

27445

RefSeq (Protein):NP_055325.2