Piccolo Antibody: HRP

• Catalog Number: orb152170
• Category: Antibodies
• Description: Rabbit polyclonal to Picollo (HRP). Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several piccolo-bassoon homology domains and C-terminal PDZ and C2 domains. In general it is found together with bassoon, a related huge multi-domain protein of the CAZ (cytoskeletal matric assembled at active zones). Piccolo is a scaffolding protein for proteins involved in endo- and exocytosis of synaptic vesicles. Piccolo has also been shown to interfere with clathrin medicated endocytosis by binding to the F-actin and dynamin binding protein Abp1..
• Target: Piccolo
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: HRP
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
• Purification: Protein A Purified
• Immunogen: Recombinant Full length Protein, BC001304.1
• UniProt ID: Q9Y6V0
• MW: 550kDa
• Tested applications: ELISA, IHC, WB
• Dilution range: WB (1:1000), IHC (1:400)
• Application notes: 1 µg/ml of SPC-197 was sufficient for detection of Piccolo in 10 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat ant rabbit IgG:HRP as the secondary antibody.
• Storage: Conjugated antibodies should be stored according to the product label
• Alternative names: ACZ antibody, Aczonin antibody, Brain-derived HLMN
• Research Area: Disease Biomarkers, Neuroscience
• Note: For research use only
• Entrez: 27445
• NCBI: NP_055325.2

Immunohistochemistry analysis using Rabbit Anti-Piccolo Polyclonal Antibody. Tissue: Muscle. Species: Mouse. Primary Antibody: Rabbit Anti-Piccolo Polyclonal Antibody at 1:400. Secondary Antibody: Alexa Fluor 488 Goat Anti-Rabbit. Counterstain: BTX (red). Localization: Selective staining of the NMJ.

Western blot analysis of Mouse, Rat brain cell lysates showing detection of Piccolo protein using Rabbit Anti-Piccolo Polyclonal Antibody. Primary Antibody: Rabbit Anti-Piccolo Polyclonal Antibody at 1:1000.