PI3KCD Antibody (N-term)

SKU: orb1428218

Description

Rabbit polyclonal antibody to PI3KCD.

Research Area

Cell Biology, Immunology & Inflammation, Immunology & inflammation, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:50~100
• Reactivity: Human
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB11627
• Immunogen: Synthetic Peptide
• Target: PIK3CD
• Molecular Weight: 119479
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform, PI3-kinase subunit delta, PI3K-delta, PI3Kdelta, PtdIns-3-kinase subunit delta, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta, PtdIns-3-kinase subunit p110-delta, p110delta, PIK3CD

Fluorescent image of Hela cell stained with PI3KCD Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PI3KCD primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). PI3KCD immunoreactivity is localized to Cytoplasm significantly.

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PI3KCD Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot analysis of anti-PI3KCD Antibody (N-term) Pab in 293 cell line lysates. PI3KCD Antibody (N-term) (arrow) was detected using the purified Pab.

Quick Database Links

Gene Symbol

PIK3CD

UniProt

O00329

RefSeq (Protein):NP_005017.3