PHYTOSPHINGOSINE

SKU: orb1940185

Description

Phytosphingosine is a phospholipid with anticancer properties. In cancer cells, Phytosphingosine induces cell apoptosis through activation of caspase 8 and translocation of Bax.

Key Properties

• CAS Number: 554-62-1
• MW: 317.514
• Purity: >98% (HPLC)
• Formula: C₁₈H₃₉NO₃
• SMILES: CCCCCCCCCCCCCC[C@@H](O)[C@@H](O)[C@@H](N)CO
• Target: Apoptosis
• Solubility: In Vitro: DMSO : 50 mg/mL (157.48 mM)

Bioactivity

• In Vitro:
  Cell Viability Assay Cell line: A549, LLC, BEAS-2B. Concentration: 1 μg/mL, 2 μg/mL, 3 μg/mL, 4 μg/mL, 5 μg/mL. Incubation time: 24 h, 48 h. Result: Significantly inhibited cell proliferation in a concentration- and time-dependent manner. Cell Cycle Analysis Cell line: A549. Concentration: 1 μg/mL, 3 μg/mL, 5 μg/mL. Incubation time: 24 h. Result: Significantly increased the proportion of G2/M phase cells. Western blot analysis. Cell line: A549. Concentration: 1 μg/mL, 3 μg/mL, 5 μg/mL. Incubation time: 24 h. Result: Up-regulated the pro-apoptotic factor Bax protein level and down-regulated the anti-apoptotic factor Bcl-2 protein level. Promoted the release of cytochrome c to up-regulate caspase 9 and caspase 3 levels, leading to PARP cleavage.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Apoptosis