Phycoerythrin Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat]

• Catalog Number: orb1674988
• Category: Antibodies
• Description: Minimum Cross Reactivity to Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins
• Species/Host: Goat
• Tested applications: FACS
• Reactivity: Rat
• Immunogen: Rat IgG whole molecule
• Antibody Type: Secondary Antibody
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm). OD566nm/OD280nm ratio: 2.0
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
• Conjugation: Unconjugated
• Storage: Store vial at 4° C prior to opening. DO NOT FREEZE. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date for properly stored material is six (6) months from date of opening.
• Buffer/Preservatives: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Note: For research use only
• Application notes: The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 µg of conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
• Expiration Date: 12 months from date of receipt.