PHOX2B Antibody (Center)

• Catalog Number: orb1938260
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB28842
• Tested applications: IF, WB
• Predicted Reactivity: C. elegans, Human, Rat
• Reactivity: Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:1000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 31621 Da
• Target: This PHOX2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-130 amino acids from the Central region of human PHOX2B.
• UniProt ID: Q99453
• NCBI: NP_003915.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Paired mesoderm homeobox protein 2B, Neuroblastoma
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Fluorescent image of U251 cell stained with PHOX2B Antibody (Center). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PHOX2B primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). PHOX2B immunoreactivity is localized to Nucleus significantly.

PHOX2B Antibody (Center) western blot analysis in mouse liver tissue lysates (35 ug/lane). This demonstrates the PHOX2B antibody detected the PHOX2B protein (arrow).