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PHOX2B Antibody (Center)
Catalog Number: orb1938260
| Catalog Number | orb1938260 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Target | This PHOX2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-130 amino acids from the Central region of human PHOX2B. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Mouse |
| Predicted Reactivity | C. elegans, Human, Rat |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| UniProt ID | Q99453 |
| MW | 31621 Da |
| Tested applications | IF, WB |
| Dilution range | IF: 1:10~50, WB: 1:1000 |
| Antibody Type | Primary Antibody |
| Clone Number | RB28842 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Paired mesoderm homeobox protein 2B, Neuroblastoma Read more... |
| Note | For research use only |
| NCBI | NP_003915.2 |
Fluorescent image of U251 cell stained with PHOX2B Antibody (Center). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PHOX2B primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). PHOX2B immunoreactivity is localized to Nucleus significantly.
PHOX2B Antibody (Center) western blot analysis in mouse liver tissue lysates (35 ug/lane). This demonstrates the PHOX2B antibody detected the PHOX2B protein (arrow).
