PHOX2B Antibody (Center)

SKU: orb1938260

Description

PHOX2B Antibody (Center)

Research Area

Neuroscience

Images & Validation

• Tested Applications: IF, WB
• Dilution range: WB - 1:500
• Reactivity: Mouse
• Predicted Reactivity: Human, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 31621 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

PMX2B

Fluorescent image of U251 cell stained with PHOX2B Antibody (Center). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PHOX2B primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). PHOX2B immunoreactivity is localized to Nucleus significantly.

PHOX2B Antibody (Center) western blot analysis in mouse liver tissue lysates (35 ug/lane). This demonstrates the PHOX2B antibody detected the PHOX2B protein (arrow).

Quick Database Links

UniProt

Q99453