Phosphotyrosine (P-Tyr) Antibody

SKU: orb388991

Description

Mouse monoclonal antibody to Phosphotyrosine

Images & Validation

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Tested Applications	FC, IF, WB
Dilution range	FACS: 0.5-1 μg/million cells, IF/ICC: 1-2 μg/ml, WB: 0.5-1 μg/ml, IP: 1-2 μg/500 μg protein Lysate, IHC-P: 1-2 μg/ml
Reactivity	All
Application Notes	Phosphotyrosine (P-Tyr)

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG2b
Clone No.	PY20
Immunogen	Phosphotyrosine conjugated to KLH
Source/Expression System	Bioreactor
Molecular Weight	Depends upon the phosphorylated target
Purification	Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Disclaimer	For research use only

Alternative Names

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P-Tyr

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Immunohistochemical staining of human Breast Carcinoma tissue using Phosphotyrosine antibody

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Protocol Information

Western Blot (IB, immunoblot)

Flow Cytometry

Immunofluorescence