Phosphotyrosine Antibody [PY20]

SKU: orb1252650

Description

Phosphotyrosine Antibody [PY20]

Research Area

Cancer Biology, Metabolism Research, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC, IP, WB
• Reactivity: All
• Application Notes:
  Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 µg/mLWB: 0.5-1 µg/mLIP: 1-2ug/500ug protein lysateIHC (FFPE): 1-2 µg/mL for 30 minutes at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Phosphotyrosine antibody to be titered up or down for optimal performance.1. No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: PY20
• Immunogen: Phosphotyrosine conjugated to KLH was used as the immunogen for this antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only