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Phosphotyrosine Antibody / P-Tyr

Catalog Number: orb749422

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb749422
CategoryAntibodies
DescriptionProtein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This antibody shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.
ClonalityMonoclonal
Species/HostMouse
IsotypeMouse IgG2b, kappa
ConjugationUnconjugated
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
PurityProtein G affinity chromatography
ImmunogenPhosphotyrosine conjugated to KLH was used as the immunogen for this antibody.
Tested applicationsFACS, IF, IHC-P, IP, WB
Dilution rangeFlow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml,Immunoprecipitation: 1-2ug/500ug protein lysate,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application notesThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Phosphotyrosine antibody to be titered up or down for optimal performance.1. No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.
Antibody TypePrimary Antibody
Clone NumberPY20
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
StorageStore the Phosphotyrosine antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Hazard InformationThis Phosphotyrosine antibody is available for research use only.
NoteFor research use only
Phosphotyrosine Antibody / P-Tyr

IHC staining of breast carcinoma with phosphotyrosine antibody (PY20). Note cell surface and cytoplasmic staining.

Phosphotyrosine Antibody / P-Tyr

SDS-PAGE Analysis of Purified, BSA-Free Phosphotyrosine Antibody (clone PY20). Confirmation of Integrity and Purity of the Antibody.

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