Phosphotyrosine Antibody / P-Tyr

• Catalog Number: orb749422
• Category: Antibodies
• Description: Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This antibody shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PY20
• Tested applications: FACS, IF, IHC-P, IP, WB
• Isotype: Mouse IgG2b, kappa
• Immunogen: Phosphotyrosine conjugated to KLH was used as the immunogen for this antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml,Immunoprecipitation: 1-2ug/500ug protein lysate,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Phosphotyrosine antibody is available for research use only.
• Storage: Store the Phosphotyrosine antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Phosphotyrosine antibody to be titered up or down for optimal performance.1. No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.
• Expiration Date: 12 months from date of receipt.

IHC staining of breast carcinoma with phosphotyrosine antibody (PY20). Note cell surface and cytoplasmic staining.

SDS-PAGE Analysis of Purified, BSA-Free Phosphotyrosine Antibody (clone PY20). Confirmation of Integrity and Purity of the Antibody.