Phosphotyrosine Antibody / P-Tyr

• Catalog Number: orb606961
• Category: Antibodies
• Description: Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This mAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2b, kappa
• Conjugation: Unconjugated
• Immunogen: Phosphotyrosine conjugated to BSA was used as the immunogen for the p-Tyr antibody.
• Tested applications: FACS, IF, IHC-P, IP, WB
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunoprecipitation: 1-2ug/500ug protein lysate,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Optimal dilution of the p-Tyr antibody should be determined by the researcher.1. No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues
• Antibody Type: Primary Antibody
• Clone Number: SPM102
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This p-Tyr antibody is available for research use only.
• Note: For research use only

IHC staining of human breast carcinoma with p-Tyr antibody (clone SPM102).