Phosphotyrosine Antibody

SKU: orb2638093

Description

Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This antibody shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.

Images & Validation

• Tested Applications: FACS, IF, IHC-P, IP, WB
• Dilution Range: Flow cytometry: 0.5-1ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml,Immunoprecipitation: 1-2ug/500ug protein lysate,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Phosphotyrosine antibody to be titered up or down for optimal performance.1. No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone No.: PY20
• Immunogen: Phosphotyrosine conjugated to KLH was used as the immunogen for this antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only