Phosphoserine Antibody

• Catalog Number: orb613989
• Category: Antibodies
• Description: Rabbit Anti-Phosphoserine Polyclonal
• Target: Phosphoserine
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: All
• Concentration: 0.25 mg/ml
• Buffer/Preservatives: PBS, 50% glycerol, 0.01% sodium azide *Storage buffer changes when conjugated
• Purification: Peptide Affinity Purified
• Immunogen: Phosphoserine conjugated to KLH, and phosvitin mixture
• Tested applications: ELISA, ICC, IF, IHC, IP, WB
• Dilution range: WB (1:500), ICC/IF (1:50), ELISA (1:250), IP (1:100)
• Application notes: 2 µg/ml of SPC-149 was sufficient for detection of phosphorylation signal in western blot analysis using human MMRU cells treated with 0.1 µM okadaic acid.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Phospho-ser Antibody, pS Antibody, pSer Antibody,
• Note: For research use only

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Phosphoserine Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphoserine Polyclonal Antibody at 1:50 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x.

Western blot analysis of Mouse Spleen lysates showing detection of Phosphoserine protein using Rabbit Anti-Phosphoserine Polyclonal Antibody. Primary Antibody: Rabbit Anti-Phosphoserine Polyclonal Antibody at 1:1000. Bands are responsive to treatment with varying long UV wavelengths: A (0), B (50), C (200), D (400), and E (treated with 0.1μM okadaic acid).